Up to 350 pmol of 5´ termini for cold phosphorylation and up to 50 pmol for hot phosphorylation.
Note: 1 µg of a 20 mer = 150 pmol of 5´ termini.
Example, to calculate the number of pmol in 1 µg of a 30 mer: 150 pmol X (20 mer/30 mer) = 100 pmol