Yes. α2-3,6,8 Neuraminidase has a very broad pH curve. Although optimum pH is around 5.5, we see excellent sialic acid removal at pH's as low as 4.5 and as high as pH 8.5. We have used α2-3,6,8 Neuraminidase at pH ranges more suitable for PNGase F (pH range 7.5-8.6).