Our Lambda DNA contains a total of 4 mutations:
    -ind1 at 37,589  C->T, which creates a Hind III site at 37,584.
   
    -Sam7 at 45,352 G->A.
Sam7 is an amber mutation in a gene involved in lysis of the bacterial cell membrane. The absence of a wild-type S gene product leads to an intracellular accumulation of infectious bacteriophage particles.

    -cI857 at 37,742 C->T.
cI857 is a temperature sensitive mutation that renders the CI gene product thermo labile.
   
    -cI857s7 strain also carries a G->A mutation at 43,082.