FAQ: What is the composition of the T4 DNA Ligase Reaction Buffer?

1X T4 DNA Ligase Reaction Buffer: 50 mM Tris-HCl, 10 mM MgCl₂, 10 mM Dithiothreitol 1 mM ATP, pH 7.5 @ 25°C. Supplied as a 10X concentrated stock.