SOB:
2% Vegetable peptone (or Tryptone)
0.5% Yeast Extract
10 mM NaCl
2.5 mM KCl
10 mM MgCl₂
10 mM MgSO₄

SOC:
SOB + 20 mM Glucose

LB agar:
1% Tryptone
0.5% Yeast Extract
0.17 M NaCl
1.5% Agar

Blue/White Screening:
X-gal 80 µg/ml
IPTG* 0.3 mM
* Omit IPTG for potentially toxic genes