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ΦX174 RF II DNA

Catalog # Concentration Size List Price Quantity Your Price
N3022L 1000 µg/ml 150 µg $500.00
$450.00
Catalog # Size List Price Your Price
N3022L 150 µg $500.00
$450.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • View sequence details
  • Commonly used cloning vector
  • Double-stranded nicked circular form of PhiX174 - relaxed form
  • Molecular Weight is 3.5 x106 Daltons
  • 5,386 base pairs in length
  • Greater then 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ⌽X174 DNA

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source
ΦX174 RF II is prepared by relaxing (nicking) ΦX174 RF I DNA in high salt at 75°C. Greater than 90% of the molecules are in the RF II form as indicated by agarose gel electrophoresis.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  ΦX174 RF II DNA N3022LVIAL -20 1 x 0.15 ml 1 mg/ml

Properties & Usage

Lac Repressor on Plasmid

0

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C



Companion Products
References
  • Sanger, F. et al. (1978). J. Mol.Biol.. 125, 225-246.
  • Freifelder, D. and Dewitt, R. (1977). Gene. 1, 385.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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