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EnGen® Sau Cas9

Catalog # Concentration Size List Price Quantity Your Price
M0654S 1 µM 90 pmol
Please Inquire
M0654T 20 µM 500 pmol $229.00
$206.10
Catalog # Size List Price Your Price
M0654S 90 pmol
M0654T 500 pmol $229.00
$206.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

EnGen® Sau Cas9 is a programmable DNA endonuclease guided by a single guide RNA (gRNA).  Targeting requires gRNA complementarity to the target site as well as a 5′NNGRRT protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence.  Cleavage by EnGen® Sau Cas9 occurs ~3 bases upstream of the PAM and leaves blunt ends.

  • Longer 5′ NNGRRT 3′ PAM sequence allows for higher specificity
  • Two nuclear localization signals for improved transport into the nucleus
  • High concentration liquid format option can be used for microinjection, electroporation and lipofection
  • Compatible with the EnGen® Mutation Detection Kit
  • Need guide RNA? NEB recommends the Horizon™ CRISPR Design Tool for designing and ordering synthetic guide RNA for EnGen Sau Cas9
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Bacterial Adaptive Immunity with CRISPR/Cas9
  • Monarch_MiniprepProtocol_VideoThumb
    NEB® TV Ep. 7 – Synthetic Biology/ CRISPR
EnGen® Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA.  Targeting requires a ~100 nucleotide guide RNA (gRNA) complementary to the target site, as well as a 5′ NNGRRT 3′ (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence.  DNA cleavage by EnGen Sau Cas9 occurs ~3 bases 5′ of the PAM.  EnGen Sau Cas9 has Simian virus 40 (SV40) T antigen nuclear localization signals (NLS) at both the N- and C-termini of the protein.

Schematic representation of Sau Cas9 nuclease sequence recognition and DNA cleavage



Product Source
EnGen® Sau Cas9 from Staphylococcus aureus is expressed as a C-terminal 6X His-tagged fusion in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  EnGen® Sau Cas9 M0654TVIAL -20 1 x 500 pmol 20 µM
  NEBuffer™ r3.1 B6003SVIAL -20 1 x 1.25 ml 10 X
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Notes
  • 20 µM is equal to ~2.54 mg/ml EnGen® Sau Cas9.  1 µM is equal to ~0.127 mg/ml.
Citations
Publications
  • Yourik, P., Fuchs, R.T., Mabuchi, M., Curcuru, J.L., Robb, G.B. (2019). Staphylococcus aureus Cas9 is a multiple-turnover enzyme. RNA. [Epub ahead of print],PubMedID: 30348755, DOI: 10.1261/rnr.067355.118
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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