Hot Start Taq DNA Polymerase

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M0495L	5000 units/ml	1000 units	$432.00		$388.80
M0495S	5000 units/ml	200 units	$110.00		$99.00

Catalog #	Size	List Price	Your Price
M0495L	1000 units	$432.00	$388.80
M0495S	200 units	$110.00	$99.00

Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity^1,2,3 and a 5´ flap endonuclease activity^4,5.

It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.

Product Source

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M0495S -20

	Hot Start Taq DNA Polymerase	M0495SVIAL	-20	1 x 0.04 ml	5,000 units/ml
	Standard Taq Reaction Buffer Pack	B9014SVIAL	-20	1 x 1.5 ml	10 X

M0495L -20

	Hot Start Taq DNA Polymerase	M0495LVIAL	-20	1 x 0.2 ml	5,000 units/ml
	Standard Taq Reaction Buffer Pack	B9014SVIAL	-20	3 x 1.5 ml	10 X

Application Features

High-specificity PCR
Routine PCR
Microarray Analysis
Colony PCR

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

Reaction Conditions

1X Standard Taq Reaction Buffer Pack

1X Standard Taq Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl₂
(pH 8.3 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
1X stabilizers
pH 7.4 @ 25°C

Heat Inactivation

Unit Assay Conditions

1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.

Companion Products

References

Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
Kaledin, A.S., Slyusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
Lawyer, F.C. et al. (1993). PCR Methods and Appl.. 2, 275-287.
Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.

Protocols

Usage Guidelines

Activity of Restriction Enzymes in PCR Buffers

Web Tools

FAQs

Citations

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Notice to Purchaser: Nucleic acid-based aptamers for use with thermophilic DNA polymerases are licensed exclusively by New England Biolabs, Inc. from SomaLogic, Inc. New England Biolabs, Inc. gives the Buyer/User a non-exclusive license to use the Hot-Start Taq DNA Polymerase for Research Use Only (RUO). Commercial use of this product may require a license from New England Biolabs, Inc. For additional information or to inquire about commercial use, please contact busdev@neb.com.

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

PRIMERSELECT™ is a trademark of DNAStar, Inc., Madison, Wisconsin, USA.

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