OneTaq® DNA Polymerase is an optimized blend of Taq and Deep Vent® DNA polymerases for use with routine and difficult PCR experiments.
OneTaq® 2X Master Mix with Standard Buffer is an optimized blend of Taq and Deep Vent® DNA Polymerases ideally suited to routine PCR applications from a variety of templates including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
The following reagents are supplied with this product:
20 mM Tris-HCl
22 mM KCl
22 mM NH4Cl
1.8 mM MgCl2
5% Glycerol
0.05% Tween® 20
0.06% IGEPAL® CA-630
0.2 mM dNTPs
25 units/ml OneTaq® DNA Polymerase
pH 8.9 @ 25°C
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Purchase of this product provides the purchaser with a non-exclusive license for research use only.
NEW ENGLAND BIOLABS®, ONETAQ® , QUICK-LOAD® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
DEEP VENT® is a trademark of New England Biolabs, Inc.
IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is registered trademark of Uniqema Americas LLC.