Afu Uracil-DNA Glycosylase (UDG)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M0279S	2000 units/ml	200 units	$128.00		$115.20

Catalog #	Size	List Price	Your Price
M0279S	200 units	$128.00	$115.20

A thermostable homolog of the E. coli Uracil-DNA Glycosylase (UDG) (1,2) from Archaeoglobus fulgidus. Afu UDG catalyzes the release of free uracil from uracil-containing DNA. Afu UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA.

Product Source

Cloned and overexpressed in E. coli.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M0279S -20

	Afu Uracil-DNA Glycosylase (UDG)	M0279SVIAL	-20	1 x 0.1 ml	2,000 units/ml
	ThermoPol^® II (Mg-free) Reaction Buffer	B9005SVIAL	-20	1 x 1.5 ml	10 X

Materials Sold Separately

ThermoPol® II (Mg-free) Reaction Buffer Pack

Notes

Afu UDG retains 50% activity in the presence of 150 mM NaCl. Afu UDG retains less than 1% activity after boiling for 30 minutes in standard reaction conditions.
Under standard reaction conditions, uracil glycosylase inhibitor (UGI) does not inhibit Afu UDG.

References

Sandigursky, M. and Franklin, W. A. (1999). CurrentBiology. 9,
Sandigursky, M. and Franklin, W. A. (2000). J. Biol.Chem.. 275,

Usage Guidelines

DNA Damage and PreCR

Selection Charts

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Change Notifications

Afu UDG has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10225518. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Effective 01/18/2024, unit definition has changed.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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