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Afu Uracil-DNA Glycosylase (UDG)

Catalog # Concentration Size List Price Quantity Your Price
M0279S 2000 units/ml 200 units $123.00
$110.70
Catalog # Size List Price Your Price
M0279S 200 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Mechanism for M1282

  • Archaeoglobus fulgidus Uracil DNA Glyocsylase
  • Thermostable monofunctional DNA glycosylase that catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil
  • Active on ss and dsDNA
  • Heat inactivation not possible
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    What are endonucleases and their applications?
A thermostable homolog of the E. coli Uracil-DNA Glycosylase (UDG) (1,2) from Archaeoglobus fulgidus. Afu UDG catalyzes the release of free uracil from uracil-containing DNA. Afu UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA.
Product Source
Cloned and overexpressed in E. coli.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Afu Uracil-DNA Glycosylase (UDG) M0279SVIAL -20 1 x 0.1 ml 2,000 units/ml
  ThermoPol® II (Mg-free) Reaction Buffer B9005SVIAL -20 1 x 1.5 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to release 60 pmol per minute of a fluorescently labeled 47 mer single-stranded DNA oligonucleotide containing a single uracil base in 30 minutes at 65°C in a total reaction volume of 50 µl in 1X Thermopol II Buffer.

Reaction Conditions

1X ThermoPol® II (Mg-free) Reaction Buffer
Incubate at 65°C

1X ThermoPol® II (Mg-free) Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
0.1 mg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 22717 daltons

Specific Activity

96 units/mg

Unit Assay Conditions

1X ThermoPol II (Mg-free) Reaction Buffer, 1 unit of Afu Uracil-DNA Glycosylase, 0.2 µg 3H-uracil DNA (104-105 cpm/µg) for 30 minutes at 65°C in a total reaction volume of 50 µl.

Materials Sold Separately
Notes
  • Afu UDG retains 50% activity in the presence of 150 mM NaCl. Afu UDG retains less than 1% activity after boiling for 30 minutes in standard reaction conditions.
  • Under standard reaction conditions, uracil glycosylase inhibitor (UGI) does not inhibit Afu UDG.
References
  • Sandigursky, M. and Franklin, W. A. (1999). CurrentBiology. 9,
  • Sandigursky, M. and Franklin, W. A. (2000). J. Biol.Chem.. 275,
Usage Guidelines
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Change Notifications

Afu UDG has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10225518. All subsequent (higher number) lots will contain rAlbumin. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Effective 01/18/2024, unit definition has changed.

 

Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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