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AluI Methyltransferase

Catalog # Concentration Size List Price Quantity Your Price
M0220S 5000 units/ml 100 units $123.00
$110.70
Catalog # Size List Price Your Price
M0220S 100 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

AluI Methyltransferase modifies the cytosine residue (C5) of the sequence AGCT.

 
Product Source
An E. coli strain that carries the cloned AluI modification gene from Arthrobacter luteus (ATCC 21606).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  AluI Methyltransferase M0220SVIAL -20 1 x 0.02 ml 5,000 units/ml
  AluI Methyltransferase Reaction Buffer B0220SVIAL -20 1 x 1 ml 10 X
  S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by AluI restriction endonuclease.

Reaction Conditions

1X AluI Methyltransferase Reaction Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 37°C

Control Protection Assay

AluI Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X AluI Methyltransferase Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by AluI Methyltransferase is determined by  the addition of 40 µl NEBuffer 1 supplemented with 10 mM MgCl2 and 10 units of AluI restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on an agarose gel.

Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

65°C for 20 minutes

Materials Sold Separately
References
  • Hoffman, J.L. (1986). Biochemistry. 25, 444-4449.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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