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TaqI Methyltransferase

Catalog # Concentration Size List Price Quantity Your Price
M0219S 10000 units/ml 1000 units $123.00
$110.70
Catalog # Size List Price Your Price
M0219S 1000 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA
  • Supplied with 10X rCutSmart™ Buffer and 32 mM S-adenosylmethionine (SAM)
TaqI Methyltransferase modifies the adenine residue (N6) of the sequence TCGA.
Product Source
An E. coli strain that carries the cloned TaqI modification gene from Thermus aquaticus.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  TaqI Methyltransferase M0219SVIAL -20 1 x 0.1 ml 10,000 units/ml
  rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X
  S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 65°C in a total reaction volume of 20 μl against cleavage by TaqI restriction endonuclease.

Reaction Conditions

1X rCutSmart™ Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 65°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Control Protection Assay

Protection Assay Conditions:
TaqI Methyltransferase is incubated with 1 µg λ DNA in 20 µl 1X rCutSmart Buffer and 80 µM S-adenosylmethionine, for 1 hour at 65°C. The extent of protection by TaqI Methyltransferase is determined by the addition of 30 µl 1X rCutSmart Buffer and 10 units of TaqI restriction endonuclease. Incubation for 15 minutes at 65°C is followed by analysis on an agarose gel.

Storage Buffer

10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 47845 daltons


Materials Sold Separately
Notes
  • TaqI Methyltransferase gives 25% activity at 37°C.
References
  • Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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