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MspI Methyltransferase

Catalog # Concentration Size List Price Quantity Your Price
M0215S 5000 units/ml 100 units $123.00
$110.70
Catalog # Size List Price Your Price
M0215S 100 units $123.00
$110.70
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

MspI Methyltransferase recognizes the same sequence as HpaII Methyltransferase, but modifies the external cytosine residue (C5) of the sequence CCGG.

MspI Methyltransferase recognizes the same sequence as the HpaII Methyltransferase, but modifies the external cytosine residue (C5) of the sequence CCGG.
Product Source
An E. coli strain that carries the cloned MspI modification gene from Moraxella species (ATCC 49670).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  MspI Methyltransferase M0215SVIAL -20 1 x 0.02 ml 5,000 units/ml
  MspI Methylase Reaction Buffer B0215SVIAL -20 1 x 1 ml 10 X
  S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by MspI restriction endonuclease.

Reaction Conditions

1X MspI Methylase Reaction Buffer
Supplement with 80 µM S-adenosylmethionine (SAM)
Incubate at 37°C

1X MspI Methylase Reaction Buffer
50 mM Tris-HCl
100 mM NaCl
5 mM β-ME
10 mM EDTA
(pH 7.5 @ 25°C)

Control Protection Assay

MspI Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X MspI Methyltransferase Reaction Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by MspI Methyltransferase is determined by the addition of 40 µl NEBuffer 2 supplemented with 10 mM MgCl2 and 5 units of MspI restriction endonuclease. Incubation for MspI at 37°C is followed by analysis on agarose gels.

Storage Buffer

50 mM Tris-HCl
50 mM NaCl
5 mM β-ME
10 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

No

Molecular Weight

Theoretical: 40397 daltons


Materials Sold Separately
References
  • Hoffman, J.L. (1986). Biochemistry. 25, 4444-4449.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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