Ph.D.™-C7C Phage Display Peptide Library Kit

Product Information

The Ph.D.-C7C Phage Display Peptide Library is based on a combinatorial library of random peptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 10⁹ electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage. 


Please click the link for Applications of the Ph.D. Phage Display Peptide Libraries.


Complexity: 1.2 x 10⁹ transformants.

This product is related to the following categories:

Discontinued (<3>

• Related Products

  Companion Products

  • Ph.D.™ Peptide Display Cloning System

  Materials Sold Separately

  • E. coli K12 ER2738

• References

  1. Sidhu, S.S. et al. (2003). Chembiochem.. 4, 14-25.
  2. Ferrer, M. et al. (1999). J. Pept. Res.. 54, 32-42.
  3. BouHamdan, M. et al. (1998). J. Biol. Chem.. 273, 8009-8016.
  4. Whaley, S.R. et al. (2000). Nature. 405, 665-668.
  5. Rozinov, M.N. and Nolan, G.P. (1998). Chem. Biol.. 5, 713-728.
  6. Rodi, D.J. et al. (1999). J. Mol. Biol.. 285, 197-203.
  7. Kraft, S. et al. (1999). J. Biol. Chem.. 274, 1970-1985.
  8. Koolpe, M. et al. (2002). J. Biol. Chem.. 277, 46974-46979.
  9. Mummert, M.E. et al. (2000). J. Exp. Med.. 192, 769-779.
  10. Hetian, L. et al. (2002). J. Biol. Chem.. 277, 43137-43142.
  11. White, S.J. et al. (2001). Hypertension. 37, 449-455.
  12. Azzazy, H.M. and Highsmith, W.E. (2002). Clin. Biochem.. 35, 425-445.
  13. Binetruy-Tournaire, R. et al. (2000). EMBO J.. 19, 1525-1533.
  14. Kragler, F. et al. (2000). EMBO J.. 19, 2856-2868.
  15. Gazouli, M. et al. (2002). J. Pharmacol. Exp. Ther.. 303, 627-632.
  16. Romanczuk, H. et al. (1999). Hum. Gene Ther.. 10, 2615-2626.
  17. Nicklin, S.A. et al. (2000). Circulation. 102,
  18. Jost, P.J. et al. (2001). FEBS Lett.. 489, 263-269.
  19. Rasmussen, U.B. et al. (2002). Cancer Gene Ther.. 9, 606-612.
  20. Tinoco, L.W. et al. (2002). J. Biol. Chem.. 277, 36351-36356.
  21. Stratmann, J. et al. (2002). J. Clin. Microbiol.. 40, 4244-4250.
  22. Mourez, M. et al. (2001). Nat. Biotechnol.. 19, 958-961.
  23. Rodi, D.J. et al. (2002). Curr. Opin. Chem. Biol.. 6, 92-96.
  24. Lee, L. et al. (2002). Arthritis Rheum.. 46, 2109-2120.
  25. Duerr, D.M. et al. (2004). J. Virol. Methods. 116, 177-180.
  26. Parmley, S.F. and Smith, G.P. (1988). Gene. 73, 305-318.
  27. Berggard, T. et al. (2002). J. Biol. Chem.. 277, 41954-41959.
  28. Chaudhary, J. et al. (2001). Am. J. Physiol. Cell Physiol.. 280, C1027-1030.
  29. Chen, L. and Sigler, P.B. (1999). Cell. 99, 757-768.
  30. Biorn, A.C. et al. (2004). Biochemistry. 43, 1928-1938.
  31. Ferrer, M. and Harrison, S.C. (1999). J. Virol.. 73, 5795-5802.

Protocols, Manuals & Usage

• Protocols

  1. M13 Titer Protocol for Ph.D. Phage Display
  2. M13 Amplifcation Protocol for Ph.D. Phage Display

• Manuals

  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls.

  • manualE8100_E8101_E8110_E8111_E8120

• Application Notes

  • Ph.D.™ Peptide Display Cloning System

FAQs & Troubleshooting

• FAQs

  1. Which of the three ready-made libraries should I choose?
  2. I am using Ph.D.™ Phage display and the amplified phage titer is low.
  3. I am using Ph.D.™ Phage Display and the phage DNA templates do not yield a readable sequence.
  4. I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
  5. I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
  6. When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
  7. When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
  8. I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
  9. Can a different bacterial strain be used with the Ph.D.™ Phage Display?
  10. Where can I find references for Ph.D.™ phage display libraries?

• Troubleshooting

  • Phage Display Troubleshooting Guide

Citations & Technical Literature

• Citations

  Product Citation Tool

  Additional Citations

  • Matochko WL, Cory Li S, Tang SK, Derda R (2014) Prospective identification of parasitic sequences in phage display screens Nucleic Acids Res; 42(3), 1784-98. PubMedID: 24217917, DOI: https://www.neb.com/en/10.1093/nar/gkt1104
  • Nguyen KT, Adamkiewicz MA, Hebert LE, Zygiel EM, Boyle HR, Martone CM, Meléndez-Ríos CB, Noren KA, Noren CJ, Hall MF (2014) Identification and characterization of mutant clones with enhanced propagation rates from phage-displayed peptide libraries Anal Biochem; 462C, 35-43. PubMedID: 24952360, DOI: https://www.neb.com/en/10.1016/j.ab.2014.06.007
  • Kaur K, Taneja NK, Dhingra S, Tyagi JS (2014) DevR (DosR) mimetic peptides impair transcriptional regulation and survival of Mycobacterium tuberculosis under hypoxia by inhibiting the autokinase activity of DevS sensor kinase BMC Microbiol; 14, 195. PubMedID: 25048654, DOI: https://www.neb.com/en/10.1186/1471-2180-14-195

Quality, Safety & Legal

• Quality Control Assays

  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

• Specifications & Change Notifications

  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

  • E8120S_v2

• Certificate of Analysis

  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

  • E8120S_v1_10007441
  • E8120S_v1_10026162
  • E8120S_v2_10043274
  • E8120S_v2_10054203
  • E8120S_v2_10062662
  • E8120S_v2_10081844
  • E8120S_v2_10089444
  • E8120S_v2_10109412
  • E8120S_v2_10121425
  • E8120S_v2_10131201
  • E8120S_v2_10169934
  • E8120S_v2_10171873
  • E8120S_v1_0341708

• Legal and Disclaimers

  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.