Ribosomal RNA (rRNA) is highly abundant in bacterial samples, and its removal is desirable in order to reveal the biological significance of less abundant transcripts. Specific enrichment of bacterial mRNAs is challenging due to their lack of poly(A) tails, and so the converse - efficient and specific removal of bacterial rRNA – is necessary.
The NEBNext® rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).
The kit is also available with RNAClean® beads.
The great majority of RNA in bacteria is ribosomal RNA (rRNA). This highly abundant RNA can conceal the biological significance of less abundant transcripts, and so its efficient and specific removal is desirable.
The NEBNext® rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to deplete rRNA (5S, 16S, and 23S) from gram-positive and gram-negative organisms. View species tested to date.
The kit is effective with both intact and degraded RNA preparations, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic).
The kit is also available with RNAClean® beads.
Features
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7850S | -20 |
NEBNext® DNase I | E7753-2VIAL | -20 | 1 x 0.015 ml | 20,000 units/ml | |
NEBNext® Thermostable RNase H | E7752-2VIAL | -20 | 1 x 0.012 ml | 5,000 units/ml | |
NEBNext® Bacterial rRNA Depletion Solution | E7851-2VIAL | -20 | 1 x 0.012 ml | ||
RNase H Reaction Buffer | E6312-2VIAL | -20 | 1 x 0.012 ml | ||
DNase I Reaction Buffer | E6315-2VIAL | -20 | 1 x 0.03 ml | ||
Nuclease-free Water | E6317-2VIAL | -20 | 1 x 0.4 ml | ||
NEBNext Probe Hybridization Buffer | E6314-2VIAL | -20 | 1 x 0.012 ml |
E7850L | -20 |
NEBNext® DNase I | E7753-3VIAL | -20 | 1 x 0.06 ml | 20,000 units/ml | |
NEBNext® Thermostable RNase H | E7752-3VIAL | -20 | 1 x 0.048 ml | 5,000 units/ml | |
NEBNext® Bacterial rRNA Depletion Solution | E7851-3VIAL | -20 | 1 x 0.048 ml | ||
RNase H Reaction Buffer | E6312-3VIAL | -20 | 1 x 0.048 ml | ||
DNase I Reaction Buffer | E6315-3VIAL | -20 | 1 x 0.12 ml | ||
Nuclease-free Water | E6317-3VIAL | -20 | 1 x 1.5 ml | ||
NEBNext Probe Hybridization Buffer | E6314-3VIAL | -20 | 1 x 0.048 ml |
E7850X | -20 |
NEBNext® DNase I | E7753-4VIAL | -20 | 1 x 0.24 ml | 20,000 units/ml | |
NEBNext® Thermostable RNase H | E7752-4VIAL | -20 | 1 x 0.192 ml | 5,000 units/ml | |
NEBNext® Bacterial rRNA Depletion Solution | E7851-4VIAL | -20 | 1 x 0.192 ml | ||
RNase H Reaction Buffer | E6312-4VIAL | -20 | 1 x 0.192 ml | ||
DNase I Reaction Buffer | E6315-4VIAL | -20 | 1 x 0.48 ml | ||
Nuclease-free Water | E6317-4VIAL | -20 | 1 x 6 ml | ||
NEBNext Probe Hybridization Buffer | E6314-4VIAL | -20 | 1 x 0.192 ml |
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