This product is intended for use with the original Oxford Nanopore Technologies SQK-LSK114 and Q-SQK-LSK109 protocols. The reagents required for the updated SQK-LSK114 protocols are provided in the NEBNext Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing (NEB #E7672).
The NEBNext DNA repair, end repair and ligation reagents recommended for use in the original Oxford Nanopore Technologies Ligation library preparation protocols are included in this product, at volumes designed for use in several protocols alongside Oxford Nanopore Technologies SQK-LSK114 & Q-SQK-LSK109 for singleplex ligation sequencing. Please note that the NEBNext Companion Module v2 for Oxford Nanopore Technologies Ligation Sequencing (NEB #E7672) is now available to accompany updated SQK-LSK114 protocols from Oxford Nanopore Technologies.
The following reagents are supplied with this product:
NEB # | Component Name | Component # | Stored at (°C) | Amount | Concentration |
E7180S | -20 |
NEBNext® FFPE DNA Repair Buffer | E7181AVIAL | -20 | 1 x 0.084 ml | ||
NEBNext® FFPE DNA Repair Mix | E7182AVIAL | -20 | 1 x 0.048 ml | ||
NEBNext® Ultra™ II End Prep Reaction Buffer | E7183AVIAL | -20 | 1 x 0.084 ml | ||
NEBNext® Ultra™ II End Prep Enzyme Mix | E7184AVIAL | -20 | 1 x 0.072 ml | ||
Quick T4 DNA Ligase | E7185AVIAL | -20 | 1 x 0.24 ml |
E7180L | -20 |
NEBNext® FFPE DNA Repair Buffer | E7181AAVIAL | -20 | 1 x 0.336 ml | ||
NEBNext® FFPE DNA Repair Mix | E7182AAVIAL | -20 | 1 x 0.192 ml | ||
NEBNext® Ultra™ II End Prep Reaction Buffer | E7183AAVIAL | -20 | 1 x 0.336 ml | ||
NEBNext® Ultra™ II End Prep Enzyme Mix | E7184AAVIAL | -20 | 1 x 0.288 ml | ||
Quick T4 DNA Ligase | E7185AAVIAL | -20 | 1 x 0.96 ml |
Component volumes tailored for use with singleplex SQK-LSK114 ligation sequencing
Simplified ordering and inventory management
Compatible with all devices: MinION®, GridION®, PromethION™, Flongle®
No more waste – no unnecessary buffers or excess reagents
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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