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NEBNext® Magnesium RNA Fragmentation Module

Catalog # Concentration Size List Price Quantity Your Price
E6150S 200 reactions $80.00
$72.00
Catalog # Size List Price Your Price
E6150S 200 reactions $80.00
$72.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
Fragmentation of RNA can be performed by enzymes, heat alone, or exposure to divalent cations at elevated temperature. This kit provides highly efficient and tunable RNA fragmentation by incubation with Magnesium ions at 94˚C.




The NEBNext® Magnesium RNA Fragmentation Module has been optimized to fragment RNA into small pieces by incubation with Magnesium ions at 94˚C. Fragment size is controlled by incubation time (Figure 1), and the reaction is ended by the addition of NEBNext Fragmentation Stop Solution.




Figure 1: Relative size distribution of eukaryotic mRNA fragments as seen using the Bioanalyzer 2100. Figure 1: Relative size distribution of eukaryotic mRNA fragments as seen using the Bioanalyzer 2100
Poly(A)+ mRNA (40ng) purified from the Universal Human Reference Total RNA (Stratagene) was fragmented in 1 X NEB Next Magnesium RNA Fragmentation Buffer for 1–5 minutes at 94°C. Fragmentation Reaction was stopped in1X NEBNext Fragmentation Stop Solution. Samples were diluted 1:10 in Nuclease-Free Water and analyzed in the Bioanalyzer 2100.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® RNA Fragmentation Buffer E6186AVIAL -20 1 x 0.4 ml 10 X
  NEBNext RNA Fragmentation Stop Solution E6187AVIAL -20 1 x 0.4 ml
Application Features
RNA Fragmentation
NEBNext® Magnesium RNA Fragmentation Module | New England Biolabs
Home PCR, qPCR & Amplification Technologies Products NEBNext® Magnesium RNA Fragmentation Module

NEBNext® Magnesium RNA Fragmentation Module

Fragmentation of RNA can be performed by enzymes, heat alone, or exposure to divalent cations at elevated temperature. This kit provides highly efficient and tunable RNA fragmentation by incubation with Magnesium ions at 94ËšC.
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Catalog https://www.neb.com/en/# Concentration Size
E6150S Not Applicable 200 reactions
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Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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