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LongAmp® Taq PCR Kit

Catalog # Concentration Size List Price Quantity Your Price
E5200S 100 reactions $196.00
$176.40
Catalog # Size List Price Your Price
E5200S 100 reactions $196.00
$176.40
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Exceptional performance with long amplicons

  • Optimized enzyme blend for amplification up to 30 kb from low complexity templates and difficult templates, such has human genomic DNA
  • 2X higher fidelity than Taq
  • Kit contains 5X Reaction Buffer for optimal performance
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Tips for Amplifying Large Amplicons
  • Monarch_MiniprepProtocol_VideoThumb
    Why is Tm Important in Primer Design?
  • Monarch_MiniprepProtocol_VideoThumb
    5 Tips for Setting Up Your PCR
  • Monarch_MiniprepProtocol_VideoThumb
    Overview of PCR
The LongAmp Taq PCR Kit features LongAmp Taq DNA Polymerase, which is a unique blend of Taq and Deep Vent® DNA Polymerases. The 3´→5´ exonuclease activity of Deep Vent® Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). LongAmp Taq DNA Polymerase offers two fold higher fidelity than Taq Polymerase alone. The LongAmp Taq PCR Kit is supplied with LongAmp Taq Reaction Buffer, which is optimized to give highest PCR yields up to 30 kb. The kit can generate a wide range of PCR products, up to 30 kb from plasmid or from human genomic DNA.


Amplification of specific sequences from human genomic DNA using LongAmp Taq DNA Polymerase. Amplicon sizes are indicated below the gel. Marker M is NEB 1 kb DNA Ladder (NEB #N3232).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  LongAmp® Taq DNA Polymerase M0323SVIAL -20 1 x 0.2 ml 2,500 units/ml
  Deoxynucleotide (dNTP) Solution Mix N0447SVIAL -20 1 x 0.2 ml 10 mM each dNTP
  LongAmp® Taq Reaction Buffer Pack B0323SVIAL -20 1 x 1.5 ml 5 X
  Magnesium Sulfate (MgSO4) Solution B1003SVIAL -20 1 x 1.5 ml 100 mM
  Nuclease-free Water B1502AVIAL -20 2 x 1.5 ml
Application Features
  • Long Range PCR
  • Colony PCR

Properties & Usage



Companion Products
References
  • Barnes, W.M. (1994). Proc. Natl. Acad. Sci. 91, 2216-2220.
  • Saiki R.K. et al. (1985). Science. 230, 1350-1354.
  • Powell, L.M. et al. (1987). Cell. 50, 831-840.
  • Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
  • Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). Nucleic Acids Res. 18, 7465.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Purchase of this product provides the purchaser with a non-exclusive license to use LongAmp® Taq PCR Kit for research purposes only. Commercial use of this product may require a license from New England Biolabs, Inc. under U.S. Patent No. 5,352,778.

LONGAMP® and NEW ENGLAND BIOLABS® are registered trademarks of New England Biolabs, Inc.
DEEP VENT® is a trademark of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas LLC.

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