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Taq PCR Kit

Catalog # Concentration Size List Price Quantity Your Price
E5000S 200 reactions $174.00
$156.60
Catalog # Size List Price Your Price
E5000S 200 reactions $174.00
$156.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

The industry standard

  • Ideal for routine PCR applications
  • Exceptional value in terms of cost per unit
  • Kit format includes optimized reagents and enables flexible experimental design for 200 PCR reactions

The Taq PCR Kit contains a sufficient supply of recombinant, highly purified Taq DNA Polymerase, PCR-qualified buffer solutions, deoxynucleotides and a broad-range, pre-mixed, ready-to-load DNA marker to perform 200 PCR reactions.

Highlights

Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Deoxynucleotide (dNTP) Solution Mix N0447SVIAL -20 1 x 0.2 ml 10 mM each dNTP
  Quick-Load® Purple 1 kb Plus DNA Ladder N0550AVIAL 4 1 x 0.2 ml 100 µg/ml
  Standard Taq Reaction Buffer Pack B9014SVIAL -20 1 x 1.5 ml 10 X
  Standard Taq (Mg-free) Reaction Buffer Pack B9015SVIAL -20 1 x 1.5 ml 10 X
  Magnesium Chloride (MgCl2) Solution B9021SVIAL -20 1 x 1.5 ml 25 mM
  Taq DNA Polymerase with Standard Taq Buffer M0273AVIAL -20 1 x 0.05 ml 5,000 units/ml

Properties & Usage



Companion Products
Materials Sold Separately
References
  • Saiki, R.K. et al. (1985). Science. 230, 1350-1354.
  • Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
  • Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
  • Lawyer, F.C. et al. (1993). PCRMethod and Appl.. 2, 275-287.
  • Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). Nucleic Acids Res.. 18, 7317-7322.
  • Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
  • Powell, L.M. et al. (1987). Cell. 50, 831-840.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.ONETAQ®, QUICK-LOAD®, NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.

IGEPAL® is a registered trademark of Rhodia Operations.
TWEEN® is a registered trademark of Uniqema Americas LLC.

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