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Luna® Cell Ready Lysis Module

Catalog # Concentration Size List Price Quantity Your Price
E3032S 100 reactions $822.00
$739.80
Catalog # Size List Price Your Price
E3032S 100 reactions $822.00
$739.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Coordinated cell lysis, RNA release, and genomic DNA removal in a fast 15-minute protocol
  • Effective cell lysis preparation from 10 to 100,000 cells across numerous cell lines
  • Minimal sample loss compared to alternative RNA extraction methods, especially at low cell input
  • Compatible with high-throughput applications: cell lysis can take place at room temperature
  • Optimal performance when paired with Luna Universal One-Step RT-qPCR kits: NEB #E3005 (dye-based) or NEB #E3006 (probe-based)
  • Complete lysis and one-step RT-qPCR workflow available: NEB #E3030 (dye-based) or NEB #E3031 (probe-based) 
 


Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps. The module coordinates cell lysis, RNA release, and genomic DNA removal simultaneously, resulting in cell lysate ready for direct RT-qPCR analysis with Luna Universal One-Step RT-qPCR kits. Similar to other Luna products, the lysis buffer includes an inert blue tracking dye for visual assistance throughout the workflow.

This table lists cell lines that are compatible with the Luna Cell Ready Lysis Module. It includes results seen using a 5-log dilution series (100,000 - 10 cells per 50 µl lysis) and subsequent detection of 1 µl of lysate in a 20 µl one-step RT-qPCR reaction. For each cell line, linear results are indicated in the final column. In addition, some insect cell lines have been tested and are compatible.

Table 1: Validated Cell Lines
 

Cell line Property Species Cell number per 50 μl lysate
A549 Adherent H. sapiens, Lung, carcinoma 10 - 100,000
HEK293 Adherent H. sapiens, Kidney 10 - 100,000
HeLa Adherent H. sapiens, Cervix, adenocarcinoma 10 - 100,000
HepG2 Adherent H. sapiens, Liver, carcinoma 10 - 1,000
NCI-H460 Adherent H. sapiens, Lung, carcinoma 10 - 100,000
SK-N-SH Adherent H. sapiens, Brain, Neuroblastoma 10 - 10,000
U2Os Adherent H. sapiens, Bone, osteosarcoma 10 - 100,000
Jurkat Suspension H. sapiens, T lymphocyte, leukemia 10 - 100,000
K-562 Suspension H. sapiens, Lymphoblast, leukemia 10 - 100,000


Figure 1: Luna Cell Ready Workflow



Coordinating the actions of DNase I and the Luna Cell Ready Protease, the Luna Cell Ready Lysis Module offers a simple workflow resulting in effective cell lysis, RNA release, and genomic DNA removal simultaneously in a 15-minute protocol. Up to 2 µl lysate (equivalent to RNA from 0.2 - 4,000 cells) can be transferred into 20 µl downstream RT-qPCR reactions.


 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Luna Cell Ready Lysis Buffer B8036AVIAL -20 2 x 1.4 ml 2 X
  Luna Cell Ready RNA Protection Reagent M0469AVIAL -20 1 x 0.25 ml 25 X
  Luna Cell Ready Protease M0473AVIAL -20 1 x 0.2 ml 25 X
  Luna Cell Ready Stop Solution B8037AVIAL -20 1 x 0.6 ml 10 X
 

DNase I (RNase-free)

 M0474AVIAL -20 1 x 0.5 ml 10 X
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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