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NEBExpress® Iq Competent E. coli (High Efficiency)

Catalog # Concentration Size List Price Quantity Your Price
C3037I 6 x 0.2 ml $290.00
$261.00
Catalog # Size List Price Your Price
C3037I 6 x 0.2 ml $290.00
$261.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

High efficiency chemically competent E. coli cells suitable for non T7 expression with enhanced reduction of basal expression.

  • Tight control of expression by laclq allows potentially toxic genes to be cloned
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Free of animal products
  • No dry ice surcharge on competent cell shipments from NEB
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Fast transformation plating with glass beads
Highlights
  • Enhanced BL21 derivative ideal for Plac, Ptac, Ptrc expression vectors
  • LacIq reduces basal expression
  • Ideal for pUC19 expression
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • Does not restrict methylated DNA (McrA-, McrBC-, EcoBr-m-, Mrr-)
  • Better control of IPTG induced expression of non T7 plasmids
  • Free of animal products
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBExpress® Iq Competent E. coli (High Efficiency) C3037IVIAL -80 6 x 0.2 ml
Features
  • Control of IPTG induced expression from Plac, Ptac,Ptrc and PT5-lacO
  • Protease deficient
Application Features

Chemically competent E. coli cells suitable for high efficiency transformation and protein expression, featuring control of IPTG induced expression with non-T7 plasmids.
DNA Effects on Transformation Efficiency and Colony Output: DNA Effects on Transformation Efficiency and Colony Output:
The optimal amount of DNA to use in a transformation reaction is lower than commonly recognized. Using clean, supercoiled pUC19, the efficiency of transformation is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single transformation reaction increase up to about 100 ng.
Effect of heat shock time on NEB Express Iq competent E. coli transformation efficiency: Effect of heat shock time on NEB Express Iq competent E. coli transformation efficiency:
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds.
Effect of DNA incubation time on NEB Express Iq competent E. coli transformation efficiency: Effect of DNA incubation time on NEB Express Iq competent E. coli transformation efficiency:
50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection Working Concentration
Ampicillin 100 µg/ml
Carbenicillin 100 µg/ml
Kanamycin 30 µg/ml
Streptomycin 25 µg/ml
Tetracycline 15 µg/ml

Shipping Notes

  • Ships on dry ice

Antibiotic Resistance

  • nit
  • cam


Companion Products
Materials Sold Separately
Notes
  • CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
  • STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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