T7 Express Competent E. coli (High Efficiency)

Catalog #	Concentration	Size	List Price	Quantity	Your Price
C2566H		20 x 0.05 ml	$342.00		$307.80
C2566I		6 x 0.2 ml	$260.00		$234.00

Catalog #	Size	List Price	Your Price
C2566H	20 x 0.05 ml	$342.00	$307.80
C2566I	6 x 0.2 ml	$260.00	$234.00

Chemically competent E. coli cells suitable for high efficiency transformation and protein expression.

Highlights

Enhanced BL21 derivative
T7 RNA Polymerase in the lac operon - no λ prophage
Deficient in proteases Lon and OmpT
Resistant to phage T1 (fhuA2)
Does not restrict methylated DNA (McrA^-, McrBC^-, EcoBr^-m^-, Mrr^-)
B Strain
Free of animal products

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

C2566H -80

pUC19 Vector	N3041AVIAL	-20	1 x 0.025 ml	50 pg/µl
SOC Outgrowth Medium	B9020SVIAL	4	1 x 25 ml
T7 Express Competent E. coli (High Efficiency)	C2566HVIAL	-80	20 x 0.05 ml

C2566I -80

pUC19 Vector	N3041AVIAL	-20	1 x 0.025 ml	50 pg/µl
SOC Outgrowth Medium	B9020SVIAL	4	1 x 25 ml
T7 Express Competent E. coli (High Efficiency)	C2566IVIAL	-80	6 x 0.2 ml

Features

Most popular T7 expression strain
Protease deficient

Application Features

Transformation of a toxic mammalian clone into E. coli hosts — A T7 expression plasmid and the same plasmid containing a gene encoding a toxic mammalian protein were transformed into each host. Comparison of the relative transformation efficiencies demonstrates that the T7 Express hosts provide the levels of control necessary for transformation of potentially toxic clones. BL21(DE3) could not be transformed with the toxic clone.

T7-controlled expression of a non-toxic protein in E. coli hosts — A T7 expression plasmid containing a gene encoding an E. coli protein was transformed into each host, grown to 0.6 OD and induced for 3 hours. Comparison of soluble extracts from uninduced (-) and induced (+) cells shows superior control of basal expression in the T7 Express hosts while maintaining high levels of induced expression.

Effect of heat shock time on T7 Express competent E. coli transformation efficiency — 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except heat shock time varied from 0 to 80 seconds..

Effect of DNA incubation time on T7 Express competent E. coli transformation efficiency — 50 μl of competent cells were transformed with 100 pg of pUC19 control DNA following the provided High Efficiency Transformation Protocol except DNA incubation time varied from 0 to 40 minutes.

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection	Working Concentration
Ampicillin	100 Âµg/ml
Carbenicillin	100 Âµg/ml
Chloramphenicol	33 Âµg/ml
Kanamycin	30 Âµg/ml
Streptomycin	25 Âµg/ml
Tetracycline	15 Âµg/ml

Shipping Notes

Ships on dry ice

Antibiotic Resistance

Companion Products

Materials Sold Separately

SOC Outgrowth Medium

Notes

CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.

Protocols

Usage Guidelines

Selection Charts

FAQs

Citations

See more details on Bioz

Additional Citations

Anton, B.P., Fomenkov, A., Raleigh, E.A. and Berkmen, M. (2016) Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents Genome Announc Mar 31;4(2),PubMedID: 27034504, DOI: 10.1128/genomeA.00230-16.

Publications

Anton, B.P., Fomenkov, A., Raleigh, E.A. and Berkmen, M. (2016). Complete Genome Sequence of the Engineered Escherichia coli SHuffle Strains and Their Wild-Type Parents Genome Announc. Mar 31;4(2),PubMedID: 27034504, DOI: 10.1128/genomeA.00230-16.

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

C2566H_I_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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