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BL21 Competent E. coli

Catalog # Concentration Size List Price Quantity Your Price
C2530H 20 x 0.05 ml $339.00
$305.10
Catalog # Size List Price Your Price
C2530H 20 x 0.05 ml $339.00
$305.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

BL21 Competent E. coli is a widely used non-T7 expression E. coli strain and is suitable for transformation and protein expression. This strain does not express the T7 RNA Polymerase.

  • Ideal for Plac, Ptac, Ptrc ParaBAD expression vectors
  • Protease deficient
  • No dry ice surcharge on competent cell shipments
  • Outgrowth medium included
  • Free of animal products
Featured Videos
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  • Monarch_MiniprepProtocol_VideoThumb
    Fast transformation plating with glass beads

 

Highlights
  • Routine non-T7 expression
  • Deficient in proteases Lon and OmpT
  • Resistant to phage T1 (fhuA2)
  • B Strain
  • Free of animal products
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  pUC19 Vector N3041AVIAL -20 1 x 0.025 ml 50 pg/µl
  SOC Outgrowth Medium B9020SVIAL 4 1 x 25 ml
  BL21 Competent E. coli C2530HVIAL -80 20 x 0.05 ml
Features
  • Routine non-T7 expression

Properties & Usage

Antibiotic for Plasmid Selection

Antibiotics for Plasmid Selection Working Concentration
Ampicillin 100 µg/ml
Carbenicillin 100 µg/ml
Chloramphenicol 33 µg/ml
Kanamycin 30 µg/ml
Streptomycin 25 µg/ml
Tetracycline 15 µg/ml

Shipping Notes

  • Ships on dry ice


Companion Products
Materials Sold Separately
Notes
  • CAUTION: This product contains DMSO, a hazardous material. Review the MSDS before handling.
  • STORAGE AND HANDLING: Competent cells should be stored at -80°C. Storage at -20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above -80°C, even if they do not thaw.
  • COMPATIBLE EXPRESSION VECTORS/PROMOTERS: Ptac, Ptrc, Plac, PlacUV5, ParaBAD, PrhaBAD, T5-lacO
    Note: Expression from vectors containing a T5-lacO promoter will not be regulated unless the expression vector encodes the lacI gene or a compatible vector expresses LacI or the host strain overproduces LacI (e.g NEB #C3037 NEB Express Iq Competent E. coli)."
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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