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Oligo d(T)25 Magnetic Beads

Catalog # Concentration Size List Price Quantity Your Price
S1419S 5 mg/ml 5 ml $509.00
$458.10
Catalog # Size List Price Your Price
S1419S 5 ml $509.00
$458.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Oligo d(T)25 is covalently coupled to 1 μm superparamagnetic particle through a linkage that is stable and leak resistant over a wide pH range, thereby permitting the small-scale isolation of mRNA from crude cell lysates and tissue. The isolation occurs through the hybridization of covalently coupled oligo d(T)25 to the poly(A) region present in most eukaryotic mRNAs. 

• Allows for direct mRNA isolation following lysis and second-round purification of previously isolated total RNA
• The magnetic separation technology is scalable and permits elution of intact mRNA in small volumes, thereby eliminating the need for precipitation of the isolated mRNA
• Beads can be reused up to three times 
• The isolated mRNA can either be eluted or the bound d(T)25 can be used as a primer in a first-strand cDNA reaction

Supplied as a 5 mg/ml suspension in phosphate buffered saline (PBS buffer) (pH 7.4), containing 0.05% Tween® 20 and 0.05% NaN3.

Recommended Buffers

Lysis/Binding Buffer: 100 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.5% Lithium Dodecyl Sulfate (LiDS), 1 mM EDTA, 5 mM DTT

Wash Buffer I: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 0.1% LiDS, 1 mM EDTA, 5 mM DTT

Wash Buffer II: 20 mM Tris-HCl, pH 7.5, 500 mM LiCl, 1 mM EDTA

Low-Salt Buffer: 20 mM Tris-HCl, pH 7.5, 200 mM LiCl, 1 mM EDTA

Elution Buffer: 20 mM Tris-HCl, pH 7.5, 1 mM EDTA

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Oligo d(T)25 Magnetic Beads S1419SVIAL 4 1 x 25 mg 5 mg/ml

Properties & Usage

Storage Buffer

0.05% Tween® 20
0.05% NaN3
1X PBS
pH 7.4 @ 25°C

Support Matrix

1 μm nonporous superparamagnetic microparticles.

Binding Capacity

1 mg of Oligo d(T)25 Beads will bind 10 µg of poly(A)+ RNA.

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Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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