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Ribonucleoside Vanadyl Complex

Catalog # Concentration Size List Price Quantity Your Price
S1402S 200 mM 10 ml $146.00
$131.40
Catalog # Size List Price Your Price
S1402S 10 ml $146.00
$131.40
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Potent inhibitor of various ribonucleases
  • Compatible with cell fractionation methods as well as sucrose-gradient centrifugations
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    Avoiding RNase Contamination
Ribonucleoside-vanadyl complex is a potent inhibitor of various ribonucleases (1,2,3). This complex is compatible with cell fractionation methods as well as sucrose-gradient centrifugations. The 200 mM stock solution is reconstituted to a green-black clear solution by incubating the sealed vial at 65°C. Once open, the entire sample should be aliquoted into smaller samples and frozen.

The ribonucleoside-vanadyl complex should be added to all buffers to a final concentration of 10 mM. The buffers should not contain EDTA since one equivalent will totally dissociate the complex.

We do not recommend the use of the vanadyl complex in cell-free translation systems and with reverse transcriptase (5). The vanadyl complex can be used in the selective degradation of DNA while preserving RNA since pancreatic deoxyribonuclease I is not inhibited (5). Removal of the ribonucleoside-vanadyl complex from the RNA can be accomplished by adding 10 equivalents of EDTA before ethanol precipitation.
Product Source
This vanadyl complex is prepared from a modification of procedures by Lienhard using all four ribonucleosides (4).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Ribonucleoside Vanadyl Complex S1402SVIAL -20 1 x 10 ml 200 mM

Properties & Usage

Shipping Notes

  • Ships on dry ice


Notes
  • Concentration of ribonucleoside-complex at 50% inhibition 2.4 mM.
  • The toxicological properties of this compound have not been fully investigated
  • Avoid contact with skin.
References
  • Gray, J.C. (1974). Arch. Biochem. Biophys.. 163, 343-348.
  • Egberts, E., Hackett, P.B. and Traub, P. (1971). Hoppe-Zeyler's Z. Physiol Chem.. 358, 475-490.
  • Leinhard, G.E., Secemski, I.I., Koehler, K.A. and Lindquist, R.N. (1971). Cold Spring Harbor Symp. Quant. Biol.. 36, 45-51.
  • Berger, S.L., Hitchcock, N.J.M., Zoon, K.C., Birkenmier, C.S., Friedman, R.M. and Chang, E.H. (1980). J. Biol. Chem.. 21, 4602-4608.
Tech Tips
  • Please, make sure this product is stored frozen until just before using or aliquotting.
  • This product is very susceptible to oxidation so it is recommended to aliquot the solution as needed and avoid refreezing any leftover solution.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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