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Random Primer Mix

Catalog # Concentration Size List Price Quantity Your Price
S1330S 60 µM 100 µl $165.00
$148.50
Catalog # Size List Price Your Price
S1330S 100 µl $165.00
$148.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Random priming is widely used in first strand cDNA synthesis.
  • Random Primer Mix is a ready-to-use, optimized mixture of 35 µM hexamers, 25 µM dT23VN, and 1 mM dNTPs. 
  • A mixture of hexamers and anchored-dT primer provides even and consistent coverage of the RNA template population across a wide range of RNA template concentration. In contrast, traditional random priming by hexamer has poor coverage of 3´ end of RNA templates (1,2,3).
  • A final concentration of 6 µM is recommended.


We recommend using Random Primer Mix for reverse transcription of the following RNA templates:

  1. RNA without poly(A) tail such as ribosomal RNAs.
  2. RNA with strong secondary structures.
  3. Partially degraded RNA samples.
  4. Target regions at 5´ end of a long messenger RNA transcript.
Figure 1: 
Figure 1
Various amounts of human spleen total RNA (2 μg to 20 pg) were reverse transcribed using ProtoScript First Strand cDNA Synthesis Master Mix Kit (NEB #E6300 ) in the presence of 6 μM Random Primer Mix (panel A) or 3.5 μM random hexamers (panel B). About 1/10th of the cDNA products were then analyzed in duplicate qPCR reactions using a pair of primers specific for the beta actin gene with DyNAmo™ FLASH SYBR® Green qPCR Master Mix on a Bio-Rad IQ5® system.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Random Primer Mix S1330SVIAL -20 1 x 0.1 ml 60 µM

Properties & Usage



Notes
  • Recommended Usage: Random Primer Mix (60 µM) contains 35 µM hexamers and 25 µM dT23VN  and 1 mM dNTPs. We recommend using Random Primer Mix at a final concentration of 6 µM.
References
  • Goff, L.A. et al. (2004). BMC Genomics. 5, 76.
  • Djikeng, A. et al. (2008). BMC Genomics. 9, 5.
  • Rashtchian, A. (1994). Genome Research. 4, 83.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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