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Random Primer 9

Catalog # Concentration Size List Price Quantity Your Price
S1254S 1 A260 units $165.00
$148.50
Catalog # Size List Price Your Price
S1254S 1 A260 units $165.00
$148.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

A mixture of random nonanucleotides used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). The primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabeled nucleotide for the appropriate non-radioactive nucleotide in the reaction mixture. Use of synthetic d(N9) primer ensures the presence of virtually all sequence combination of nanomer primers which results in equally labelled DNA of high specific activity (1,2).

Oligolabeling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).

Sequence

5' d(N9) 3' [N = A,C,G,T]

 
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Random Primer 9 S1254SVIAL -20 1 x 1 A260 units

Properties & Usage



Companion Products
Notes
  • 12.4 nmol of S1254 are supplied.
References
  • Feinberg, A.P. and Vogelstein, B. (1983). Anal.Biochem.. 132, 6-13.
  • Feinberg, A.P. and Vogelstein, B. (1984). Anal.Biochem.. 137, 266-267.
  • Grunstein, M. and Hogness, D.S. (1975). Proc. Natl. Acad. Sci. USA. 72, 3961-3965.
  • Southern, E.M. (1975). J. Mol. Biol.. 98, 503-517.
  • Smith, G.E. and Summers, M.D. (1980). Anal.Biochem.. 109, 123-129.
  • Hasse, A. et al. (1984). MethodsVirol.. 7, 189-226.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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