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Random Primer 6

Catalog # Concentration Size List Price Quantity Your Price
S1230S 1 A260 units $165.00
$148.50
Catalog # Size List Price Your Price
S1230S 1 A260 units $165.00
$148.50
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Random Primer 6 is a mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA
  • Ensures the presence of virtually all sequence combinations of hexamer primers
  • Used to screen gene libraries
  • Used to probe northern and southern blots
  • Used for in situ hybridizations
  • Sequence: 5' d(N6 ) 3' [N=A,C,G,T]
This mixture of random hexanucleotides is used to prime DNA synthesis in vitro along multiple sites of denatured template DNA (1). This primer-template complex is a suitable substrate for DNA Polymerase I (Klenow Fragment). The newly synthesized complementary DNA is "oligo-labelled" by substituting any radiolabelled nucleotide for the appropriate nonradioactive nucleotide in the reaction mixture. Use of synthetic d(N)6 primer ensures the presence of virtually all sequence combination of hexamer primers which results in equally labelled DNA of high specific activity (1,2). Oligolabelling by this method generates probes which can be used to screen gene libraries (3), probe Southern and Northern blots (4,5), and for in situ hybridizations (6).
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Random Primer 6 S1230SVIAL -20 1 x 1 A260 units

Properties & Usage



Notes
  • For use in standard Random Priming Reaction, dissolve 33 µg of (NEB #S1230S) in 80 µl of water or 10X labeling buffer.
  • 18.6 nmol of S1230 are supplied.
References
  • Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 132,
  • Feinberg, A.P. and Vogelstein, B. Anal. Biochem.. 137,
  • Grunstein, M. and Hogness, D.S. Proc. Natl. Acad. Sci. USA. 72,
  • Southern, E.M. J. Mol. Biol.. 98,
  • Smith, G.E. and Summers, M.D. Anal. Biochem.. 109,
  • Hasse, A. et al. Methods Virol.. 7,
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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