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5-methyl-dCTP

Catalog # Concentration Size List Price Quantity Your Price
N0356S 10 mM 1 µmol $120.00
$108.00
Catalog # Size List Price Your Price
N0356S 1 µmol $120.00
$108.00
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Cytosine modification at carbon 5 (C5) represents an important epigenetic modification. It is also believed to be the starting substrate for the Ten-Eleven Translocation (TET) family of enzymes and their associated oxidation pathways.

  • 5-methyl-dCTP (2'-deoxy-5-methylcytidine 5'-triphosphate) offers the ability to enzymatically make defined, fully methylated, cytosine-substituted DNA, which can be used for a variety of biochemical and cellular applications
  • 5-methyl-dCTP is supplied as a 10 mM solution in Milli-Q™ water. Nucleotide concentration is determined by measurements of absorbance at 260 nm.

 

 

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  5-methyl-dCTP N0356SVIAL -20 1 x 0.1 ml 10 mM

Properties & Usage

Supplied As

Milli-Q water as a triethylammonium salt

Molecular Weight

Theoretical: 481.1 daltons


Companion Products
Notes
  • Supplied In:
    Milli-Q™ water as an ammonium salt at (pH 7.0)
  • Formula:
    C10H15N3O13P3 (free acid)
  • Diluent Compatibility:
    Can be diluted using sterile distilled water, preferably Milli-Q™ water, or can be diluted using sterile TE (10 mM Tris-HCl, 1 mM EDTA (pH 7.5)).
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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