New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB

We are transitioning to our new website at www.neb.com, which will go live on April 7th, 2025.

On April 2nd at 8:00 PM ET, www.neb.ca e-commerce functionality will be disabled for the website transition. If you need to place your order between April 2nd at 8:00 pm ET to April 6th, then please call 1-800-387-1095 or email orders.ca@neb.com.

The transition to the new website will not affect our shipping schedule. If you're a registered user, all your account details, including order history and shipping details will be safely transferred to the new site.

M13KO7 Helper Phage

Catalog # Concentration Size List Price Quantity Your Price
N0315S 1 x 10<sup>11</sup> pfu/ml 1.80 ml $194.00
$174.60
Catalog # Size List Price Your Price
N0315S 1.80 ml $194.00
$174.60
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
M13KO7 is an M13 derivative, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication (1). M13KO7 is able to replicate in the absence of phagemid DNA. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid is packaged preferentially and secreted into the culture medium.This allows easy production of single-stranded phagemid DNA for mutagenesis or sequencing.
Product Source
13KO7 Phage supernatant is isolated from infected E. coli ER2738 by a standard procedure.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  M13KO7 Helper Phage N0315SVIAL -20 2 x 0.9 ml 1 x 1011 pfu/ml
M13KO7 Helper Phage | New England Biolabs

M13KO7 Helper Phage

M13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is packaged preferentially and secreted into the culture medium. This allows easy production of single-stranded phagemid DNA.
Blog_ContextualAd
Catalog https://www.neb.com/en/# Concentration Size
N0315S 1 x 1011 pfu/ml 1.8 ml
Please enter a quantity for at least one size

  • Product Information

    M13KO7 is an M13 derivative, with the origin of replication from P15A and the kanamycin resistance gene from Tn903 both inserted within the M13 origin of replication (1). M13KO7 is able to replicate in the absence of phagemid DNA. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid is packaged preferentially and secreted into the culture medium.This allows easy production of single-stranded phagemid DNA for mutagenesis or sequencing.

    Product Source

    13KO7 Phage supernatant is isolated from infected E. coli ER2738 by a standard procedure.
    This product is related to the following categories:
    Other Products,
    Phage Display Products,
    cDNA Synthesis & Reverse Transcriptases Products
    This product can be used in the following applications:
    Phage Display,
    PCR

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB https://www.neb.com/en/# Component Name Component https://www.neb.com/en/# Stored at (°C) Amount Concentration

    • Properties & Usage

      Affinity Tag

      Other

      Supplied As

      Liquid

      Sequence Files

      Fasta GenBank

    • Product Notes

      1. NEB does not recommend the use of M13KO7 as a cloning vector. For cloning peptides displayed on M13 phage, we recommend the Ph.D.™ Peptide Display Cloning System.

    • References

      1. Vieira, J. and Messing, J. (1987). R. Wu and L. Grossman(Ed.), Methods Enzymol.. 153, 3-11. San Diego: Academic Press.
      2. Sambrook, J. and Russell, D.W. (2001). Molecular Cloning: A laboratory Manual, (3rd ed.),. 3.17-3.32. Cold Spring Harbor: Cold Spring Harbor Laboratory Press..

  • Protocols, Manuals & Usage

  • Tools & Resources

  • Citations & Technical Literature

    • Citations

      Product Citation Tool

  • Quality, Safety & Legal

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly. If you have any questions, please contact Customer Support at freezers@neb.com or 1-800-632-5227 x 8.

Continue

Choose your country

If you don&https://www.neb.com/en/#39;t see your country above, please visit our international site

Loading Spinner

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.

Sign In



Notes
  • NEB does not recommend the use of M13KO7 as a cloning vector. For cloning peptides displayed on M13 phage, we recommend the Ph.D.™ Peptide Display Cloning System.
References
  • Vieira, J. and Messing, J. (1987). R. Wu and L. Grossman(Ed.), Methods Enzymol.. 153, 3-11. San Diego: Academic Press.
  • Sambrook, J. and Russell, D.W. (2001). Molecular Cloning: A laboratory Manual, (3rd ed.),. 3.17-3.32. Cold Spring Harbor: Cold Spring Harbor Laboratory Press..
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Top