New England Biolabs Canada
Expand menu
Sign In or Sign UpMy NEB
1

Phusion® Hot Start Flex 2X Master Mix

Catalog # Concentration Size List Price Quantity Your Price
M0536L 2 X 500 reactions $1,231.00
$1,107.90
M0536S 2 X 100 reactions $309.00
$278.10
Catalog # Size List Price Your Price
M0536L 500 reactions $1,231.00
$1,107.90
M0536S 100 reactions $309.00
$278.10
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

  • 50X higher fidelity than Taq
  • Hot start formulation reduces non-specific amplification
  • Master mix format for ease of use and optimized performance
High Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error-rate > 50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerases available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.

Phusion Hot Start Flex 2X Master Mix offers robust high fidelity performance and room temperature reaction setup in a convenient master mix format. The addition of an aptamer-based inhibitor allows room temperature reaction setup. The convenient master mix formulation is supplied at a 2X concentration and contains dNTPs and Mg++, requiring only the addition of primers and DNA template for robust amplification. Reactions can also be optimized using the provided DMSO solution.
Product Source
An E. coli strain that carries the Phusion DNA Polymerase gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Phusion® Hot Start Flex 2X Master Mix M0536SVIAL -20 2 x 1.25 ml 2 X
  DMSO B0515AVIAL -20 1 x 0.5 ml 100 %
  Phusion® Hot Start Flex 2X Master Mix M0536SVIAL -20 10 x 1.25 ml 2 X
  DMSO B0515AVIAL -20 2 x 0.5 ml 100 %
Application Features
  • High-specificity PCR
  • Cloning
  • Long or Difficult Amplification
  • High-Throughput PCR

Properties & Usage

Heat Inactivation

No

Unit Assay Conditions

25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2 , 1 mM β-mercaptoethanol, 200 µM dNTPs including[3H]-dTTP and 15 nM primed M13 DNA.

Companion Products
Notes
  • Phusion Hot Start Flex DNA Polymerase is unlike other enzymes and care must be taken when designing cycling protocols. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.
  • Product specifications for individual components in the Phusion Hot Start Flex 2X Master Mix are available separately.
References
  • Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.
  • Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Notice to Customers: Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific.

Phusion® is a registered trademark and property of Thermo Fisher Scientific.

Notice to Purchaser: Limited License. The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patent owned by Bio-Rad Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication to the purchaser by the purchase of this product.
NEW ENGLAND BIOLABS® and Q5® are registered trademarks of New England Biolabs, Inc.
PHUSION® is a registered trademark and property of Thermo Fisher Scientific.
THERMO SCIENTIFIC® is a registered trademark of Thermo Fisher Scientific.

Other Products You May Be Interested In
 Top