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Phusion® High-Fidelity PCR Master Mix with HF Buffer

Catalog # Concentration Size List Price Quantity Your Price
M0531L 2 X 500 reactions $1,132.00
$1,018.80
M0531S 2 X 100 reactions $292.00
$262.80
Catalog # Size List Price Your Price
M0531L 500 reactions $1,132.00
$1,018.80
M0531S 100 reactions $292.00
$262.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

For high speed and high performance PCR. Manufactured and quality-controlled at New England Biolabs, Thermo Scientific® Phusion® High-Fidelity DNA Polymerase offers both high fidelity and robust performance.

  • 50X higher fidelity compared to Taq
  • Robust reactions - maximal success with minimal optimization
  • Master mix format with HF buffer ideal for a wide range of AT/GC content
High-Fidelity DNA Polymerases are important for applications in which the DNA sequence needs to be correct after amplification. Phusion High-Fidelity DNA Polymerase offers both high fidelity and robust performance, and thus can be used for all PCR applications. Its unique structure, a novel Pyrococcus-like enzyme fused with a processivity-enhancing domain, increases fidelity and speed. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult amplicons. With an error rate >50-fold lower than that of Taq DNA Polymerase and 6-fold lower than that of Pyrococcus furiosus DNA Polymerase (1), Phusion is one of the most accurate thermostable polymerase available. Phusion DNA Polymerase possesses 5´→ 3´ polymerase activity, 3´→ 5´ exonuclease activity and will generate blunt-ended products.

Phusion High-Fidelity PCR Master Mix with HF Buffer is a 2X master mix consisting of Phusion DNA Polymerase, deoxynucleotides and reaction buffer that has been optimized and includes MgCl2. All that is required is the addition of template, primers and water.

Fidelity assays were performed using a lacI-based method modified from Frey & Suppman, 1995.
A 3.8 kb fragment was amplified from 50 ng of Jurkat gDNA using different polymerases. Reactions were carried out according to the manufacturer's recommended conditions. Extension times are indicated (in minutes). Ladder L is a 1 kb DNA Ladder.
Product Source
An E. coli strain that carries the Phusion DNA Polymerase gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Phusion® High-Fidelity PCR Master Mix with HF Buffer M0531SVIAL -20 2 x 1.25 ml 2 X
  DMSO B0515AVIAL -20 1 x 0.5 ml 100 %
  Phusion® High-Fidelity PCR Master Mix with HF Buffer M0531SVIAL -20 10 x 1.25 ml 2 X
  DMSO B0515AVIAL -20 2 x 0.5 ml 100 %
Features
  • Robust Reactions - Maximal success with minimal optimization.
  • Extreme Fidelity - > 50X greater than Taq
  • High Speed - Dramatically reduced extension times (10X faster than Pfu)
  • High Yield - Increased product yield using minimal amount of enzyme.
  • Versatile - Can be used for routine per as well as long or difficult templates.
Application Features
  • PCR
  • Cloning
  • Long or Difficult Amplification
  • High-Throughput PCR

Properties & Usage

Heat Inactivation

No

Unit Assay Conditions

25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2 , 1 mM β-mercaptoethanol, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

Companion Products
Notes
  • Products specifications for individual components in the Phusion High-Fidelity PCR Master Mix with HF Buffer are available separately.
References
  • Frey, B. and Suppman, B. (1995). Biochemica. 2, 34-35.
  • Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290.
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.

Licenses/Patents/Disclaimers:
Notice to purchaser: Limited license.
The purchase price of this product includes a limited, non-transferable license under U.S. and foreign patents owned by BIO-RAD Laboratories, Inc., to use this product. No other license under these patents is conveyed expressly or by implication by purchase of this product.

PHUSION® is a registered trademark and property of Thermo Fisher Scientific.
THERMO SCIENTIFIC® is a registered trademark and property of Thermo Fisher Scientific.

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