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Q5® High-Fidelity DNA Polymerase

Catalog # Concentration Size List Price Quantity Your Price
M0491L 2000 units/ml 500 units $665.00
$598.50
M0491S 2000 units/ml 100 units $168.00
$151.20
Catalog # Size List Price Your Price
M0491L 500 units $665.00
$598.50
M0491S 100 units $168.00
$151.20
Catalog #
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*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

Q5® High-Fidelity DNA Polymerase sets a new standard for both fidelity and robust performance. With the highest fidelity amplification available (~280 times higher than Taq), Q5 results in ultra-low error rates. Q5 is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. 

Working with uracil-containing DNA templates or using dUTP? Learn about Q5U Hot Start High-Fidelity DNA Polymerase (NEB #M0515).

  • Highest fidelity amplification (~280X higher than Taq)
  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Also available: Q5 Hot Start standalone polymerase (NEB #M0493) and master mix formats (NEB #M0492, M0494)
Featured Videos
View Video Library
  • Monarch_MiniprepProtocol_VideoThumb
    Important Tips for Q5® Polymerase
  • Monarch_MiniprepProtocol_VideoThumb
    Why Choose Q5® High-fidelity Polymerase?
  • Monarch_MiniprepProtocol_VideoThumb
    Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing
  • Monarch_MiniprepProtocol_VideoThumb
    Why is Tm Important in Primer Design?
  • Monarch_MiniprepProtocol_VideoThumb
    Tips for Amplifying Large Amplicons
  • Monarch_MiniprepProtocol_VideoThumb
    How to Amplify GC-rich DNA
  • Monarch_MiniprepProtocol_VideoThumb
    5 Tips for Setting Up Your PCR
  • Monarch_MiniprepProtocol_VideoThumb
    Overview of PCR

 

Q5® High-Fidelity DNA Polymerase is a high-fidelity, thermostable DNA polymerase with 3´→ 5´ exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning and can be used for long or difficult amplicons. Q5 High-Fidelity DNA Polymerase is supplied with an optimized buffer system that allows robust amplification regardless of GC content. The 5X Q5 Reaction Buffer contains 2 mM Mg++ at final (1X) reaction concentrations and is recommended for most routine applications. For GC-rich targets (≥ 65% GC), amplification can be improved by the addition of the 5X Q5 High GC Enhancer. Q5 High-Fidelity DNA Polymerase is unlike typical, lower fidelity PCR enzymes. To determine the optimal annealing temperatures for a given set of primers, use of the NEB Tm Calculator is highly recommended.

M0493 
Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase. All reactions were conducted using 30 cycles of amplification and visualized by microfluidic LabChip® analysis.
Product Source
An E. coli strain that carries the Q5 High-Fidelity DNA Polymerase gene.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  Q5® High-Fidelity DNA Polymerase M0491SVIAL -20 1 x 0.05 ml 2,000 units/ml
  Q5® Reaction Buffer Pack B9027SVIAL -20 1 x 1.5 ml 5 X
  Q5® High GC Enhancer B9028AVIAL -20 1 x 1.5 ml 5 X
  Q5® High-Fidelity DNA Polymerase M0491LVIAL -20 1 x 0.25 ml 2,000 units/ml
  Q5® Reaction Buffer Pack B9027SVIAL -20 4 x 1.5 ml 5 X
  Q5® High GC Enhancer B9028AVIAL -20 4 x 1.5 ml 5 X
Application Features
  • High-fidelity PCR
  • Cloning
  • Long or Difficult Amplification
  • High-throughput PCR

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 74°C.

Unit Assay Conditions

25 mM TAPS-HCl (pH 9.3 @ 25°C), 50 mM KCl, 2 mM MgCl2, 1 mM β-mercaptoethanol, 200 μM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.

Companion Products
Citations
Additional Citations
  • Harish Nag Kankipati, Marta Rubio-Texeira, Dries Castermans, George Diallinas, Johan M Thevelein (2015) Sul1 and Sul2 Sulfate Transceptors Signal to Protein Kinase A upon Exit of Sulfur Starvation. J Biol Chem 290, 10430-46.PubMedID: 25724649, DOI: 10.1074/jbc.M114.629022
  • Amin Zargar, David N Quan, Milad Emamian, Chen Yu Tsao, Hsuan-Chen Wu, Chelsea R Virgile, William E Bentley (2015) Rational design of 'controller cells' to manipulate protein and phenotype expression. Metab Eng ,PubMedID: 25908186, DOI: 10.1016/j.ymben.2015.04.001
  • Yonghe Zhang, Huiming Huang, Shanshan Xu, Bo Wang, Jianhua Ju, Huarong Tan, Wenli Li (2015) Activation and enhancement of Fredericamycin A production in deepsea-derived Streptomyces somaliensis SCSIO ZH66 by using ribosome engineering and response surface methodology. Microb Cell Fact 14, 64.PubMedID: 25927229, DOI: 10.1186/s12934-015-0244-2
  • Silva-Herzog E, McDonald EM, Crooks AL, Detweiler CS. (2015) Physiologic Stresses Reveal a Salmonella Persister State and TA Family Toxins Modulate Tolerance to These Stresses PLoS One 12,PubMedID: 26633172, DOI: 10.1371
  • Jun Wu, Daiji Okamura, Mo Li, Keiichiro Suzuki, Chongyuan Luo, Li Ma, Yupeng He, Zhongwei Li, Chris Benner, Isao Tamura, Marie N Krause, Joseph R Nery, Tingting Du, Zhuzhu Zhang, Tomoaki Hishida, Yuta Takahashi, Emi Aizawa, Na Young Kim, Jeronimo Lajara, Pedro Guillen, Josep M Campistol, Concepcion Rodriguez Esteban, Pablo J Ross, Alan Saghatelian, Bing Ren, Joseph R Ecker, Juan Carlos Izpisua Belmonte (2015) An alternative pluripotent state confers interspecies chimaeric competency. Nature ,PubMedID: 25945737, DOI: 10.1038/nature14413
  • Longhai Dai, Can Liu, Yueming Zhu, Jiangsheng Zhang, Yan Men, Zeng Yan, Yuanxia Sun (2015) Functional Characterization of Cucurbitadienol Synthase and Triterpene Glycosyltransferase Involved in Biosynthesis of Mogrosides from Siraitia grosvenorii. Plant Cell Physiol ,PubMedID: 25759326, DOI: 10.1093/pcp/pcv043
  • Yang YJ, Han YY, Chen K, Zhang Y, Liu X, Li S, Wang KQ, Ge JB, Liu W, Zuo J. (2015) TonEBP modulates the protective effect of taurine in ischemia-induced cytotoxicity in cardiomyocytes Cell Death DisPubMedID: 26673669, DOI: 10.1038
  • Yuan Xue, Jossef Osborn, Anand Panchal, Jay L Mellies (2015) The RpoE Stress Response Pathway Mediates Reduction of the Virulence of Enteropathogenic Escherichia coli by Zinc. Appl Environ Microbiol 81, 3766-74.PubMedID: 25819956, DOI: 10.1128/AEM.00507-15
  • Christine Henke, Pamela L Strissel, Maria-Theresa Schubert, Megan Mitchell, Claus C Stolt, Florian Faschingbauer, Matthias W Beckmann, Reiner Strick (2015) Selective expression of sense and antisense transcripts of the sushi-ichi-related retrotransposon - derived family during mouse placentogenesis. Retrovirology 12, 9.PubMedID: 25888968, DOI: 10.1186/s12977-015-0138-8
  • Binyamin D Berkovits, Christine Mayr (2015) Alternative 3' UTRs act as scaffolds to regulate membrane protein localization. Nature ,PubMedID: 25896326, DOI: 10.1038/nature14321
  • Wang XJ, Zhang XJ, Hu W, Zhang TY, Wang SQ (2014) A simple and efficient strategy for the de novo construction of greater-than-genome-length hepatitis B virus replicons J Virol Methods 207, 158-62.PubMedID: 25025817, DOI: 10.1016/j.jviromet.2014.07.009
  • Vidhyadhar Nandana, Sushant Singh, Abhay Narayan Singh, Vikash Kumar Dubey (2014) Procerain B, a cysteine protease from Calotropis procera, requires N-terminus pro-region for activity: cDNA cloning and expression with pro-sequence. Protein Expr Purif 103C, 16-22.PubMedID: 25173974, DOI: 10.1016/j.pep.2014.08.003
  • Martin Kostovcik, Craig C Bateman, Miroslav Kolarik, Lukasz L Stelinski, Bjarte H Jordal, Jiri Hulcr (2014) The ambrosia symbiosis is specific in some species and promiscuous in others: evidence from community pyrosequencing. ISME J ,PubMedID: 25083930, DOI: 10.1038/ismej.2014.115
  • Bert De Rybel, Milad Adibi, Alice S. Breda, Jos R. Wendrich, Margot E. Smit, Ondej Novk, Nobutoshi Yamaguchi, Saiko Yoshida, Gert Van Isterdael, Joakim Palovaara, Bart Nijsse, Mark V. Boekschoten, Guido Hooiveld, Tom Beeckman, Doris Wagner, Karin Ljung, Christian Fleck, Dolf Weijers (2014) Integration of growth and patterning during vascular tissue formation in Arabidopsis Science 345, 1255215.PubMedID: 25104393, DOI: 10.1126/science.1255215
  • Xin Duan, Arjun Krishnaswamy, Irina De la Huerta, Joshua R Sanes (2014) Type II Cadherins Guide Assembly of a Direction-Selective Retinal Circuit. Cell 158, 793-807.PubMedID: 25126785, DOI: 10.1016/j.cell.2014.06.047
  • Lieve Naesens, Luke Guddat, Dianne Keough, André B.P. van Kuilenburg, Judith Meijer, Johan Vande Voorde and Jan Balzarini (2013) ROLE OF HUMAN HYPOXANTHINE GUANINE PHOSPHORIBOSYLTRANSFERASE IN ACTIVATION OF THE ANTIVIRAL AGENT T-705 (FAVIPIRAVIR) Mol Pharmacol 87247.PubMedID: 23907213
  • Anastassia Voronova Erin Coyne, Ashraf Al Madhoun, Joel V. Fair, Neven Bosiljcic, Catherine St-Louis, Grace Li, Sherry Thurig, Valerie A. Wallace, Nadine Wiper-Bergeron, and Ilona S. Skerjanc (2013) Hedgehog Signaling Regulates MyoD Expression and Activity J Biol Chem 288(6), 4389–4404.PubMedID: 23266826
  • Hicham Bouabe, Klaus Okkenhaug (2013) A Protocol for Construction of Gene Targeting Vectors and Generation of Homologous Recombinant Embryonic Stem Cells Methods Mol Biol 1064, 337-354.PubMedID: 23996269
  • Wilber Quispe-Tintaya, Ryan R White, Vasily N Popov, Jan Vijg, Alexander Y Maslov (2013) Fast mitochondrial DNA isolation from mammalian cells for next-generation sequencing Biotechniques 55(3), 133-6.PubMedID: 24003945, DOI: 10.2144/000114077
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

This product is covered by one or more patents.

This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.

For additional information or to inquire about commercial use, please contact busdev@neb.com.



Q5® is a registered trademark of New England Biolabs, Inc.
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