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NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer

Catalog # Concentration Size List Price Quantity Your Price
B6117S 10 X 0.40 ml $52.00
$46.80
Catalog # Size List Price Your Price
B6117S 0.40 ml $52.00
$46.80
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • Designed for NEBNext second strand synthesis reactions where alternative concentrations or compositions of deoxynucleotides are required.
  • 10X concentration
The 10X Second Strand Synthesis (dNTP-free) Reaction Buffer is optimized for use with NEBNext® Second Strand Synthesis Enzyme Mix, when alternative concentrations or compositions of deoxynucleotides are required.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® Second Strand Synthesis (dNTP-free) Reaction Buffer B6117SVIAL -20 5 x 0.08 ml 10 X

Properties & Usage

1X Buffer Components

20 mM Tris-HCl
12 mM (NH4)2SO4
5 mM MgCl2
0.16 mM β-NAD
pH 7.5@25°C



Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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