Protocol for decapping of total RNA (NEB #M0463)
| Component | Concentration | Final Concentration | Volume |
|---|---|---|---|
| Total RNA | 1-5 µg/reaction | ||
| yDcpS | 200 units/µl | 4 units/µl | 1µl |
| yDcpS Reaction Buffer | 10X | 1X | 5 µl |
| Nuclease Free Water | Up to 50 µl | ||
| Final Volume | 50 µl |
Incubate at 37˚C for 1 hour.
Inactivate yDcpS by incubation at 70˚C for 15 minutes.
Proceed with RNA analysis.