Protocol for LAMP Reactions with Tte UvrD Helicase (#M1202)
Prepare 25 μL reactions as follows:
| WarmStart LAMP Master Mix (E1700, 2X) | 12.5 μL |
| LAMP Fluorescent Dye (50X) | 0.5 μL |
| Primers (10X) | 2.5 μL* |
| Tte UvrD Helicase | 0.5 – 1 μL (10 – 20 ng) |
| DNA or RNA sample | various |
| Nuclease-Free Water | to 25 μL |
* We recommend 1.6 μM each FIP and BIP, 0.2 μM each F3 and B3, and 0.4 μM each Loop F and Loop B primers. See the manual for the WarmStart LAMP Kit (DNA & RNA), NEB #E1700, for more details.