Protocol for use with EGCase I (P0773)
- Prepare a 20 µl reaction as follows:
Glycosphingolipid 2 nmol EGCase I Buffer (10X) 2 µl EGCase I enzyme 6 mU (1 μl) H2O up to 20 µl*
- Incubate at [37°C] for 16 hours
*Scale larger reaction volumes proportionally.