Denaturing PAGE/Urea or Denaturing Agarose Gel (NEB #B0363)

Protocol

1. Add sample to an equal volume of RNA Loading Dye, (2X). Mix well.
2. Heat at 65–70°C for 5–10 minutes to denature RNA.
3. While heating the samples, setup the gel box and flush urea out of the wells with running buffer using a large tip.
4. Load samples.