Optimal activity is achieved with the recommended Isothermal Amplification Buffer supplemented with 1 mM ATP or in the WarmStart LAMP 2X Master Mix (NEB #E1700). But Tte UvrD Helicase is active in other buffers, and relative activity in some other potential buffers is shown below:
| Buffer | Relative Activity |
|---|---|
| NEBuffer 1.1 | 100% |
| NEBuffer 2.1 | 100% |
| NEBuffer 3.1 | 75% |
| CutSmart® Buffer | 100% |
| Isothermal Amplification Buffer II | 10% |
| ThermoPol® Buffer | 100% |
| WarmStart LAMP Master Mix | 100% |