Purity of eluted RNA samples can be quickly assessed by reviewing OD ratios collected during routine spectrophotometry. Pure RNA typically has an A_260/280 of 1.9–2.1, and an A_260/230 of 2.0–2.2. Many factors can influence these values such as the use of a proper reference blank solution, the buffer pH, and contaminants such as protein, buffer salts, ethanol, etc.