See substrates listed in Table 1 below, which was adapted from: Albrecht, S. et al. (2016) Analytical Chemistry 88, 4795-4802.

Table 1. Substrate specificity of recombinant Endoglycoceramidase I
The table below lists the quantities of glycans released from glycolipid standards (2 nmol) using 2 mU R. triatomea rEGCase I following incubation at 37°C for 16 hours.

Substrate	Glycolipid Structure	Hydrolysis %
Gangliosides
GM3	Neu5Acα2-3Galβ1-4Glcβ1-1'Cer	100
GM1a	Galβ1-3GalNAcβ1-4(Neu5Acα2-3) Galβ1-4Glcβ1-1'Cer	100
FGM1	Fucα1-2Galβ1-3GalNAcβ1-4 (Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer	100
GD3	Neu5Acα2-8Neu5Acα2-3Galβ1-4 Glcβ1-1'Cer	100
GD1a	Neu5Acα2-3Galβ1-3GalNAcβ1-4 (Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer	100
GD1b	Galβ1-3GalNAcβ1-4(Neu5Acα2-8 Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer	100
GT1b	Neu5Acα2-3Galβ1-3GalNAcβ1-4 (Neu5Acα2-8Neu5Acα2-3)Galβ1-4Glcβ1-1'Cer	100
Globosides
Gb4	GalNAcβ1-3Galα1-4Galβ1-4Glcβ1-1'Cer	100
Cerebrosides
GlcCer	Glcβ1-1'Cer	100
GalCer	Galβ1-1'Cer	5-10
Sulfatide	HSO3-3Galβ1-1'Cer	0
Psychosine	Galβ1-1'sphingosine	0
LacCer	Galβ1-4Glcβ1-1'Cer	100