The activity of Taq DNA Ligase in other buffers is as follows:
NEBuffer™ r2.1: 10,000 units/ml
NEBuffer™ r3.1: 8-10,000 units/ml
rCutSmart™ Buffer: 12-16,000 units/ml
Full activity is achieved using unique Taq DNA Ligase Buffer, supplied with the enzyme: 40,000 units/ml.