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5-hydroxymethyluridine DNA Kinase

Catalog # Concentration Size List Price Quantity Your Price
M0659S 20000 units/ml 1000 units $117.00
$105.30
Catalog # Size List Price Your Price
M0659S 1000 units $117.00
$105.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca
  • 5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA
  • This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
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    What are Enzymes for Innovation?
5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA.

Specificity
Product Source
The 5-hydroxymethyluridine DNA Kinase enzyme is isolated from a strain of E. coli which contains the htdk gene from Pseudomonas aeruginosa bacteriophage M6.
Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  5-hydroxymethyluridine DNA Kinase M0659SVIAL -20 1 x 0.05 ml 20,000 units/ml
  T4 DNA Ligase Reaction Buffer B0202SVIAL -20 1 x 1 ml 10 X
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

References
  • Weigele, P.R., et al. (2017). FASEB. 31,
Citations
Additional Citations
  • Vaníková, Z., Janousková, M., Kambová, M., Krásny, and Hocek, M (2019) Switching transcription with bacterial RNA polymerase through photocaging, photorelease and phosphorylation reactions in the major groove of DNA Chem Sci DOI: 10.1039/c9sc00205g
Quality Control Assay
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
Safety Data Sheets
Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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