NudC Pyrophosphatase

Catalog #	Concentration	Size	List Price	Quantity	Your Price
M0607S	10 µM	250 pmol	$119.00		$107.10

Catalog #	Size	List Price	Your Price
M0607S	250 pmol	$119.00	$107.10

Decaps RNA that contains ADP-containing, non-canonical initiating nucleotides (including NAD⁺/NADH caps), resulting in ligatible monophosphate at the 5′ end.
Hydrolyzes NADH and NAD⁺ into AMP and reduced nicotinamide monophosphate nucleoside (NMNH), and AMP and nicotinamide monophosphate nucleoside (NMN⁺), respectively.
Hydrolyzes diphosphate dinucleotides and metabolic co-factors containing an ADP moiety such as AppA, FAD, Coenzyme A, etc.
Useful for NAD⁺ decapping or deNADding when NAD⁺ is the 5′ initiating nucleotide of RNA.
Useful for detection and identification of RNA containing non-canonical initiating nucleotides using ligation-based methods such as 5′ RACE and RNA-seq.

This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.

NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD⁺- and NADH-capped RNA, generating a ligatible 5′ monophosphate on the RNA (NAD⁺ decapping or deNADding) ⁽¹⁾. Deletion of the nudC gene has been shown to increase the fraction of NAD⁺-capped RNA in E. coli ⁽²⁾.

NudC has also been shown to hydrolyze small molecules containing an ADP moiety such as NADH, NAD⁺, AppA, ADP-ribose, ADP-glucose and metabolic co-factors such as FAD, Coenzyme A and Acetyl CoA at varying efficiency ^{(3 and unpublished results)}. With its activity on various dinucleotides and metabolic co-factors, NudC may potentially be used to identify RNA carrying non-canonical caps by pyrophosphorolysis of the cap and analysis of the product nucleotide, or identifying the resulting 5' monophosphate end of the RNA using 5^’ ligation-based methods such as RNA-seq or 5’ RACE ⁽⁴⁾.

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

M0607S -20

NudC Pyrophosphatase	M0607SVIAL	-20	1 x 0.025 ml	10 µM
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Dithiothreitol (DTT)	B1222AVIAL	-20	1 x 0.5 ml	100 mM

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products

NEBuffer 3.1

References

Frick D.N. and Bessman M.J. (1995). J. Biol. Chem. 270, 1529-1534.
Cahová, H. et al. (2015). Nature. 519, 374-377.
Höfer K. et al. (2016). Nat Chem Biol. 12, 730-734.
Vvedenskaya I.O., et al (2018). Mol Cell. 70, 553-564.e9.

Protocols

Protocol for using NudC in decapping RNA containing non-canonical initiating nucleotides (NEB #M0607)

FAQs

Quality Control Assay

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Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M0607S_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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