BsmBI-v2

This product replaces BsmBI (NEB #R0580).

Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments).

We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free.

Catalog #	Concentration	Size
R0739L	10000 units/ml	1000 units
R0739S	10000 units/ml	200 units

Catalog #	Concentration	Size
R0739L	10000 units/ml	1000 units
R0739S	10000 units/ml	200 units

Time-Saver™ qualified for digestion in 5-15 minutes
Used in Golden Gate Assembly
Type IIS restriction enzymes recognize asymmetric DNA sequences and cleave outside of their recognition sequence
Supplied with 1 vial of Gel Loading Dye, Purple (6X) (NEB #B7024)
NEB has developed convenient kits (using BsmBI-v2 and BsaI-HFv2) for performing Golden Gate Assembly.
Can be used with NEBridge^® Ligase Master Mix for Golden Gate Assembly
Restriction Enzyme Cut Site: CGTCTC

Featured Videos

View Video Library

Golden Gate Assembly Workflow
Golden Gate Assembly Domestication Tutorial
Listen to DAD Informatics tools and NEB enzymes to enable complex one-pot Golden Gate Assemblies
Listen to DAD (Data-optimized Assembly Design) when constructing high-complexity Golden Gate Assembly targets
Golden Gate Assembly Tool Tutorial
NEB^® TV Ep. 15 – Applications of Restriction Enzymes
TIME-SAVER™ Protocol for Restriction Enzyme Digests
Restriction Enzyme Digest Problem: Too Many DNA Bands
Why is My Restriction Enzyme Not Cutting DNA?
Function and Application of Type IIS Restriction Enzymes

Product Source

An E. coli strain that carries the modified cloned BsmBI gene from Bacillus stearothermophilus B61. BsmBI-v2 is an engineered and improved version of BsmBI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

R0739S -20

BsmBI-v2	R0739SVIAL	-20	1 x 0.02 ml	10,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

R0739L -20

BsmBI-v2	R0739LVIAL	-20	1 x 0.1 ml	10,000 units/ml
NEBuffer™ r3.1	B6003SVIAL	-20	1 x 1.25 ml	10 X
Gel Loading Dye, Purple (6X)	B7024AVIAL	25	1 x 0.5 ml	6 X

An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

Companion Products

Materials Sold Separately

Gel Loading Dye, Purple (6X), no SDS

Notes

BsmBI-v2 is an isoschizomer of Esp3I

Protocols

Usage Guidelines

Selection Charts

Web Tools

FAQs

Citations

See more details on Bioz

Quality Control Assay

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

R0739S_L_v1

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety Data Sheets

Legal And Disclaimer

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.