New England Biolabs Canada

NEBNext® Multiplex Oligos for Illumina® (Dual Index Primers Set 1)  

Catalog # Concentration Size
E7600S 96 reactions
Catalog # Concentration Size
E7600S 96 reactions

NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification. This kit includes 8 i5 index primers and 12 i7 index primers for dual indexing.

For additional information on available indices and formats for multiplexing, please refer to the NEBNext Multiplex Oligos Selection Chart.

  • High efficiency adaptor ligation
  • Minimized adaptor-dimer formation
  • Includes index primers for library multiplexing
  • Increased sample identification specificity



Incorporating a novel hairpin loop structure, the NEBNext® Adaptor ligates with increased efficiency to end-repaired, dA-tailed DNA. The loop contains a U, which is removed by treatment with USER™ Enzyme (a mix of UDG and Endo VIII), to open up the loop and make it available as a substrate for PCR. During PCR, barcodes can be incorporated by use of the NEBNext index primers, thereby enabling multiplexing. This kit includes 8 i5 index primers and 12 i7 index primers.

NEBNext Oligos can be used with NEBNext products, and with other standard Illumina-compatible library preparation protocols.

Single primer options are also available, in 12- and 96- index formats (NEB #E7335, NEB #E7500, NEB #E7710, NEB #E7730, NEB #E6609). A methylated version of the NEBNext Adaptor is also available for use with bisulfite sequencing protocols (NEB #E7535).

Dual Index Workflow
Dual Index Workflow

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext Adaptor for Illumina E7601AVIAL -20 1 x 0.96 ml
  USER® Enzyme E7602AVIAL -20 1 x 0.288 ml
  NEBNext i501 Primer E7603AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i502 Primer E7604AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i503 Primer E7605AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i504 Primer E7606AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i505 Primer E7607AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i506 Primer E7608AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i507 Primer E7609AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i508 Primer E7610AVIAL -20 1 x 0.06 ml 10 µM
  NEBNext i701 Primer E7611AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i702 Primer E7612AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i703 Primer E7613AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i704 Primer E7614AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i705 Primer E7615AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i706 Primer E7616AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i707 Primer E7617AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i708 Primer E7618AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i709 Primer E7619AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i710 Primer E7620AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i711 Primer E7621AVIAL -20 1 x 0.04 ml 10 µM
  NEBNext i712 Primer E7622AVIAL -20 1 x 0.04 ml 10 µM

Properties & Usage

Materials Required but not Supplied

For the list of additional materials required, please check the manual for your NEBNext Library Prep Kit.

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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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