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NEBNext® Primers for Epigenetics (Unique Dual Index Set 3)

Catalog # Concentration Size List Price Quantity Your Price
E3404S 96 reactions $647.00
$582.30
Catalog # Size List Price Your Price
E3404S 96 reactions $647.00
$582.30
Catalog #
Qty:
 
*On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca

NEBNext® Primers for Epigenetics (Unique Dual Index Set 3) includes 96 unique pairs of i5 and i7 index primers optimized for NEBNext epigenetics workflows, including NEBNext E5hmC-seq™, with Illumina® sequencing. The primers enable incorporation of unique index pairs during library amplification and have been designed to minimize index hopping.

  • Optimized for the NEBNext epigenetics workflows, including E5hmC-seq
  • Addresses the “barcode hopping” issue seen with some Illumina sequencing instruments
  • Increases sample identification specificity with unique dual index primer pairs

Indices are incorporated during PCR using these Unique Dual Index primer pairs that have been optimized for NEBNext epigenetics workflows, including E5hmC-seq. These primers enable multiplexing and have been designed to minimize index hopping. They are supplied as premixed i5 and i7 8-base index primers in 96 wells of a plate that has a pierceable foil seal for easy, single use.

The index sequences from the NEBNext Primers for Epigenetics (Unique Dual Index Set 3) (NEB #E3404S) and the 24-reaction NEBNext Primers for Epigenetics (Unique Dual Index Set 2B) (NEB #E3392S) are compatible, and can be combined to multiplex up to 120 reactions.

Please refer to the NEBNext Enzymatic 5hmC-seq Kit manual (NEB #E3350) for use of the NEBNext Primers for Epigenetics.

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  NEBNext® Primers for Epigenetics (Unique Dual Index Set 3) E3404AVIAL -20 1 x 5 μl/well 10 µM
An exception occurred during the operation, making the result invalid. Check InnerException for exception details.

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The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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